Production of Citric Acid by Trichoderma viride Isolated from Soil in Keffi, Nigeria Using Glucose Enhanced Substrates

Aim: This study was aimed at the production of citric acid by Trichoderma viride (T. viride) isolated from soil in keffi, Nigeria, using glucose enhanced substrate.

Place and Duration of the Study: Department of Microbiology Faculty of Natural and Applied Sciences Nassarawa State University Keffi, Nigeria, between April and June 2017.

Methodology: Trichoderma viride was isolated from soil in Keffi and identified using standard microbiology methods. Two types of glucose production media were prepared by following standard fermentation conditions. The citric acid produced was estimated using Gas Chromatography/Mass Spectrometry (GC/MS) method respectively.

Results: The maximum citric acid production of 12.03±0.31g/l was obtained at pH 6.0 with glucose with soybeans cake by T. viride and on sugar concentration of 160 g/l 15.17±3.01 g/l. The fermentation broth containing glucose and soy beans cake has the highest production of citric acid on both fermentation parameters tested respectively.

Conclusion: Different fermentation conditions such as pH and sugar concentration substrate enhanced on the production of citric acid. This study showed that pH 6.0 with glucose with soybeans cake has highest citric acid production and at sugar concentration of 160 g/l with glucose and soybeans cake produced the highest citric acid by T. viride.