Raeisi, Adham and Ramezani, Mostafa and Ravazadeh, Hossein and A. Taher, Mahdi (2019) Chromatographic Behaviour of Antibiotics on Thin Layers of Zeolite. Journal of Pharmaceutical Research International, 30 (4). pp. 1-8. ISSN 2456-9119
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Abstract
Introduction: Antibiotics analysis is performed by many methods such as spectrophotometry, fluorimetry, polarography, and high-performance liquid chromatography. This analysis doesn't require derivatization but requires expensive equipment and extensive preparation. When more than one antibiotic is present in a formulation, interactions may occur between the drugs that must be separated before measurement. Thin-layer chromatography is a useful technique for identifying antibiotics because of the low cost, high speed, and low servicing. Silica gel adsorbents have often been used as adsorbents in all thin-layer chromatography studies. In this study, zeolite was used as an adsorbent in thin- layer chromatography with high selectivity.
Materials and Methods: The chromatographic behaviour of amoxicillin, ampicillin, cefazolin, cefixime, ceftriaxone, cefalexin, and penicillin was studied for the first time on a thin layer of zeolite with mobile, organic, and organic- organic phases.
Discussion: The best separation of ceftriaxone from amoxicillin, ampicillin, cefazolin, cefixolin, cefalexin, and penicillin on a thin layer of zeolite using methanol as the mobile phase. The distance and rise time are 12 cm and 110 minutes, respectively.
Conclusion: The results of the present study showed that using the current method, the selectivity of one antibiotic from other components as well as two-component andthree-component adsorption was obtained. Quantitative identification of antibiotics was also performed in multicomponent mixtures after selection of appropriate isolates.
Item Type: | Article |
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Subjects: | Souths Book > Medical Science |
Depositing User: | Unnamed user with email support@southsbook.com |
Date Deposited: | 24 Apr 2023 06:57 |
Last Modified: | 19 Sep 2024 09:52 |
URI: | http://research.europeanlibrarypress.com/id/eprint/650 |