Comparative Analysis of Sexual Dimorphic Ecdysteroid Regulating Mblk & E74 Gene Expression Levels by Real Time PCR from Galleria mellonella

The Greater wax moth Galleria mellonella belongs to the family of Pyralidae which cause great damage to the honey bee yield worldwide, a major insect model. The honey bee cultivation places are most habitat of this insect. Most the organisms of insects rely on their olfactory system to detect and analyse the sensible nature in the environment and it is mostly useful in the concept of behavioural emotions includes mating, host defence and other sensory related molecular functions. This organism is the only member of the genus Galleria and also it is found in most the places in the world. The larvae of Galleria mellonella is used as commercial product as proving the food for predatory insects. The pathogenicity and toxicology testing were making the researchers easy by replacing the use of small mammals for in-vivo and in-vitro studies. The neurological mechanism of this insect is still unclear.

The ecdysone induced protein is unique protein with various functions, types, diversity in different organisms. From the previous study indicate that the ecdysteroid regulated gene - E74 & mushroom like large body specific protein expressed in kenyon cells of brain and was involved in olfactory, neurological behaviour of insects, embryonic and larval development. The gene expression pattern was predicted by using real time PCR. From the analyzed results, that the genes MBLK & E74 genes were expressed in Galleria mellonella which allow us to better understand of neurological disorders by using this organism as a conventional animal model.