Bacteriology and Physicochemical Analysis of Borehole Water in Some Hostels of a Tertiary Institution in Port Harcourt, Nigeria

Several factors influence the quality of water for human consumption. Most of these factors are more of anthropogenic origin than nature and thus exercises an untold health effect on the end-users of such water body. Bacteriology and Physicochemical Analysis of Borehole Water in Some Hostels of a Tertiary Institution in Port Harcourt, Nigeria. Water samples were collected from six (6) hostels for each study period, and subjected to standard laboratory procedures to analyze for parameters such as pH, nitrite, nitrate, dissolved oxygen (DO), electrical conductivity (EC), Phosphate, as well as standard procedures for antibiotics susceptibility and molecular characterization. Result of the physicochemical parameters showed that the parameters ranged from 5.26±3.53 mg/L to 7.61±0.01 mg/L forpH; 0.61±0.01 to 2.56±0.01 mg/L for Dissolved Oxygen (DO); 96±1.41 μS/cm to 503.5±0.71μS/cm for electrical conductivity; 4.56±0.03 mg/L to 20.3±0.01 mg/L for nitrate; 0.003±0mg/L to 0.03±0mg/L for nitrite; 0.12±0mg/L to 0.17±0mg/L for phosphate. Statistical evaluation showed there was a significant difference (p < 0.05) between the mean values of physicochemical parameters studied, except for pH that recorded no statistical difference (p > 0.05). From the study, 5.6% of Staphylococcus species were resistant to Pefloxacin and Rocephin, while 5.9% of Bacillus species were resistant to Ampiclox. Similarly, 3.33% of Salmonella species were resistant to Augmentin, while the rest of the gram negative isolates were either susceptible or within an intermediate range of susceptibility to the antibiotics tested. Molecular characterization of some bacterial species isolated had pairwise identity with Klebsiella pneumonia strain CP27 and Escherichia coli strain RIZHAO 498.1, Staphylococcus haemolyticus strain SH1. The study has shown that the physicochemical water quality variables were all within the WHO limits, with bacterial species having high percentage susceptibility to the antibiotics tested. Regular water quality monitoring is however recommended to ensure continuous access to water of good quality in hostels within the university community.