Overexpression of the CBF2 transcriptional Activator Enhances Oxidative Stress Tolerance in Arabidopsis Plants

Porat, Ron and Sharabi-Schwager, Michal and Samach, Alon (2011) Overexpression of the CBF2 transcriptional Activator Enhances Oxidative Stress Tolerance in Arabidopsis Plants. International Journal of Biology, 3 (2). pp. 94-105. ISSN 1916-9671

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Abstract

The C-repeat/dehydration-responsive-element binding factor genes (CBF1-3) are transcriptional activators
involved in governing plant responses to low temperatures; their overexpression enhances plant frost tolerance.
We found that overexpression of CBF2 in Arabidopsis enhanced oxidative stress tolerance as compared with
wild-type plants, an effect that was manifested in: increased seed germination rates on Petri dishes containing
H2O2; delayed leaf senescence following incubation with H2O2; and delayed wilting and senescence after
spraying whole plants with paraquat, a generator of superoxide radicals. Transcript profiling analysis using the
Affymetrix ATH1 genome array revealed that overexpression of CBF2 did not affect expression of reactive
oxygen-scavenging genes but rather, remarkably enhanced expression of oxidative-stress-responsive
transcription factor genes. Overall, the present findings suggest that overexpression of CBF2 in Arabidopsis
enhances oxidative stress tolerance, most likely via activation of a network interaction among stress-related
transcription-factor genes.

Item Type: Article
Subjects: Souths Book > Biological Science
Depositing User: Unnamed user with email support@southsbook.com
Date Deposited: 29 May 2023 06:34
Last Modified: 02 Oct 2024 07:25
URI: http://research.europeanlibrarypress.com/id/eprint/1016

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